DNA Sequence as an essential discipline of medicine and technology involves dynamic subsets and methods of multi-discipline areas like the incorporation of biochemical methodology in order to determine the exact arrangement of the many biochemical components such as thymine, adenine, and the nucleotide base in a single component known as the nucleic acid (DNA oligonucleotide).

The Growth of DNA Sequence Method in the Face of New Technology

Relatively, the DNA sequencing and the methods involved on it have originated from the very complex and taxing gel electrophoresis technique, which involves a tedious process of separating the DNA, the RNA, or the molecular proteins with the application of electrical current in a gel matrix. Tedious and taxing as it, many experts believe that the gel electrophoresis technique was not an efficient method that prompted many scientists to come up with a newer technique.

At present, the method that initiated a better DNA sequencing is based on an automated protocol employing dye labeling and recognition in the capillary-e (or electrophoresis). This method allows the quick sequencing of two types of genes (the genomes and the transcriptomes) all at the same time making it a lot efficient.

In the early days, the DNA Sequence method was relatively known as the RNA sequencing. This is technically a lot easier to perform compared to DNA sequencing. One of the earliest completed sequencing using this method was identified, performed, and published by the world renowned science scholar Walter Fiers and his associates at the University of Ghent. The scholar contribution of these people in the field of DNA and RNA sequencing was first released in the years 1972 and 1976.

Prior to the 1972-1976 published material of Fiers, three equally renowned scientists made an exploration on DNA sequence method. In 1970, Sanger of England and Gilbert and Maxam of Harvard explored the use of various DNA sequence methods that resulted to a unified method known as the Wandering-Spot Analysis. However, because of the complexity in the usage of this DNA sequence method, the Wandering-Spot analysis was rather short-lived.

Sanger, in his commitment to come up with a much better method created his own team and worked on developing a newer and simpler method. His work was published in 1975 and instantly became the better choice by many experts. The Chain-Termination method of Sanger and associates was relatively the method used by many DNA experts.

The Rebuttal of Gilbert and Maxam's DNA Sequencing Method

Maxam and Gilbert thought that the method used by Sanger (the Chain-Termination) was rather tedious and inaccurate because with the improved and modified Maxam and Gilbert DNA Sequence method, the modification of chemical components of the DNA and the succeeding cleavage at a particular DNA base, then there can be a better and much improved DNA sequencing result.

The Challenges that DNA Sequence Methods Face

One of the major challenges that DNA sequencing methods are facing is the presence of poor and low quality of the first 40 DNA bases and continuously becoming poorer and lower in quality as it reaches the next DNA 900 bases. The presence of advanced technology, it can trim down the poorness in the quality by employing some software packages and highly specialized computer systems.

George Edmondson is an experienced writer specializing on DNA Sequencing. To learn more about DNA Sequencing, you can visit http://www.dnasequenceinfo.com.